Receiving an error in the logs - 18059, Severity: 20, State: 1.

Hello,
I'm using SQL Server 2008 Sp3

Lately I'm having errors in the SQL Logs:

Error: 18059, Severity: 20, State: 1.
The connection has been dropped because the principal that opened it subsequently assumed a new security context, and then tried to reset the connection under its impersonated security context. This scenario is not supported. See "Impersonation Overview" in Books Online.

I have find via PROFILER the relevant originated server and application.
I googled it and found someone that says that we need to change the connection pooling in the application's Connection String.

The link:
http://nishantrana.wordpress.com/2008/12/12/the-connection-has-been-dropped-because-the-principal-that-opened-it-subsequently-assumed-a-new-security-context-and-then-tried-to-reset-the-connection-under-its-impersonated-security-context-this-s/

Below is the relevant Web.Config file that in charge on the connection string:

<?xml version="1.0"?><appSettings><add key="Main.ConnectionString" value="Data Source=db-002\db2;Initial Catalog=APPDB;TRUSTED_CONNECTION=YES"/><add key="ConnectionHash" value="==QMr1meolHO3YWdTNFOzcTO"/><add key="Database" value="[APPDB]"/><add key="UseApprole" value="True"/></appSettings>

I changed it to the following in our TEST env:

<?xml version="1.0"?><appSettings><add key="Main.ConnectionString" value="Data Source=db-002\db2;Initial Catalog=APPDB;TRUSTED_CONNECTION=YES;Pooling=false"/><add key="ConnectionHash" value="==QMr1meolHO3YWdTNFOzcTO"/><add key="Database" value="[APPDB]"/><add key="UseApprole" value="True"/></appSettings>

I've reset the application, services and IIS.
Checked the application and it works.

For some time I didn't receive these errors anymore, but after 3 hours they reverted.

Can anyone give me an idea what can I do?
Thanks in advance,
Roni.